Recurring Failures in In Vitro Fertilization
In the normal fertilization process, there are 3 primary barriers
in the woman's body that are intended to sort the good sperm cells from those
that are less normal and which can cause difficulties in fertilization and
formation of a sustainable fetus:
1. Cervical mucus that is
2. The small aperture
between the uterus and fallopian tube that the sperm cell must find and the
swimming the sperm cell against the natural flow of fluids from the fallopian
tube toward the uterus.
3. The cumulus oophorous that surrounds the ovary and thick ovarian
These obstacles cause a situation wherein out of approximately 100
million sperm cells that are naturally inserted into the female duct, only
about 50 sperm cells will reach the ovarian wall and try to penetrate it. These
cells are in a kind of competition between them as to which one will penetrate the
ovarian wall first, with the first one penetrating into the ovarian cavity, the
most talented one, activating a new interior wall the
size of the ovary in order to prevent the additional sperm cells from
The IVF process is intended to achieve pregnancy in which the
sperm cells are unable to meet with the ovary in a natural manner or
alternatively, they meet, but they lack the ability to penetrate it. Therefore,
the indications for performing IVF are:
Mechanical obstruction in the female duct that prevents passage of
the sperm cells to the ovary.
Low spermatozoa – a low quantity of sperm cells that are unable to
meet the demands of selection that are activated in the female duct.
Weak movement of sperm cells that prevents them from reaching the
ovary and can cause problems in penetration of the ovary.
Improper sperm cell structure that prevents achievement of viable
In such situations, performance of classic IVF is recommended,
wherein the ovaries aspirated from the woman's ovaries are placed in the same
petri dish together with approximately 100,000 moving sperm cells. The
proximity between gemmates can produce a situation wherein one of the sperm
cells that were placed in the dish successfully penetrates the ovarian wall and
produces normal fertilization.
In the event that there is no fertilization in classic IVF,
micromanipulation IVF (ICSI) will be recommended, wherein a single sperm cell
is forcibly injected into the ovary. The disadvantage of this method is that
this action prevents the sperm cell from undergoing any kind of natural selection, so it is possible that
the cell will be abnormal and not bring about a viable fetus. In such a
situation, the partners will suffer recurring failures in IVF.
In order to overcome this drawback, we have developed at our
laboratory early sorting of the sperm cells under high magnification (with a
microscope that magnifies more than 6,000 times) so that we will be able to
achieve viable pregnancy even through the micromanipulation method.
This method, known as “the Bartoov Method” or IMSI in scientific
terms, requires much knowledge in order to select the appropriate cell so that
it will resemble as much as possible the one chosen by natural selection
generated by the ovarian sheath.
The Bartoov Method (IMSI)
In this method, the sperm sample is scanned
under a high magnification microscope (x 6,000) in real time on the day that
the woman's ovaries are aspirated, and the sperm cells having the best chances
of achieving pregnancy based on their structure are isolated. These cells are
sent to the IVF unit, where they are injected into the woman's ovaries.
The method is based on 80 different
parameters of sperm cell head structure. These parameters were developed by
Prof. Bartoov following research of almost 20 years into the use of transmission
(TEM) and scanning electron microscopy (SEM), which enable magnification of
20,000. For clinical use, a counter photomicroscope was developed that magnifies
by 6,000 times aided by DIC optics.
With this method, we double the
pregnancy rate and significantly reduce the rate of miscarriages that occur in
The implementation of this method
requires great patience on the part of the technicians, who need to choose from
thousands of sperm cells those isolated cells that are morphologically normal.
This procedure takes an average of approximately 4 hours of painstaking work by
means of the special microscope.